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. 2020 Oct 22;26:100335. doi: 10.1016/j.jbo.2020.100335

Fig. 5.

Fig. 5

FOXD3-AS1 functioned in OS by targeting ZCCHC3. (A) RT-qPCR detected ZCCHC3 expression in MG-63 cells after the transfection with pcDNA3.1 or pcDNA3.1/ZCCHC3. Student’s t test. (B) Wound healing assay (scale bar = 200 μm) measured the migration of MG-63 cells under diverse conditions. One-way ANOVA. (C) Transwell assay (scale bar = 200 μm) tested the invasion of MG-63 cells with different transfections. One-way ANOVA. (D) Western blot measured E-cadherin and N-cadherin protein levels in indicated MG-63 cells. (E) IF assay (scale bar = 50 μm) estimated the fluorescence intensity of E-cadherin and N-cadherin in indicated MG-63 cells. One-way ANOVA. **P < 0.01.