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. 2020 Oct 22;26:100335. doi: 10.1016/j.jbo.2020.100335

Fig. 7.

Fig. 7

ELF1 activated FOXD3-AS1 transcription in OS. (A) RT-qPCR analyzed the efficiency of ELF1 overexpression (Student’s t test) and knockdown (one-way ANOVA) in both MG-63 and HOS cells. (B) RT-qPCR detected FOXD3-AS1 expression in OS cells under ELF1 upregulation or downregulation. Student’s t test. (C) DNA motif of ELF1 from JASPAR. (D) Two binding sites for ELF1 in FOXD3-AS1 promoter were predicted by JASPAR. (E) Luciferase reporter assay measured the luciferase activity of indicated FOXD3-AS1 promoter in MG-63 and HOS cells after the transfection of pcDNA3.1/ELF1 or pcDNA3.1. Student’s t test. (F) ChIP assay verified the binding between ELF1 and FOXD3-AS1 promoter in these two OS cells. Student’s t test. (G-H) Wound healing (scale bar = 200 μm) and transwell assays (scale bar = 200 μm) measured the migration and invasion of indicated MG-63 cells. One-way ANOVA. (I) Western blot analyzed the protein levels of E-cadherin and N-cadherin in indicated MG-63 cells. (J) IF assay (scale bar = 50 μm) determined the fluorescence intensity of E-cadherin and N-cadherin in MG-63 cells under diverse transfections. One-way ANOVA. **P < 0.01.