Table 1.

Primers set for the real-time PCR amplification.

Virus	Target region	Nucleotide	Primers	Sequences	Fragment length
HERV-H* (23)	env	5388–5407	HERV-H Fw	5′-TTC ACT CCA TCC TTG GCT AT-3′	129
		5498–5517	HERV-H Rev	5′-CGT CGA GTA TCT ACG AGC AAT-3′
HERV-K§	env	93656–93675	HERV-K Fw	5′-CAC AAC TAA AGA AGC TGA CG-3′	167
		93804–93823	HERV-K Rev	5′-CAT AGG CCC AGT TGG TAT AG-3′
HERV-K# (24)	pol	4212–4232	HERV-K-pol Fw	5′-ATC CCA AAA GAT TGG CCT TTA-3′	166
		4357–4378	HERVK-pol Rev	5′-TTA AGC ATT CCC TGA GGT AAC A-3′
HERV-P∧	env	364–382	HERV-P Fw	5′-CAA GAT TGG GTC CCC TCA C-3′	208
		554–572	HERV-P Rev	5′-CCT ATG GGG TCT TTC CCT C-3′

^*HERV-H env: 100% similarity with Chr.2q24.3.

^§HERV-K env: 100% similarity with Chr.12q14.1, and 6q14.1.

^#HERV-K pol: 100% similarity with Chr. 1q22/23.3; 3q27.2/24/13.2; 5q33.3;5p13.3; 6q14.1; 7q22.2; 7p22.1a/1b; 8q24.3c; 11q22.1; 12q14.1; 19q11; 19p12b/12d/12e; Xq21.33.

^∧HERV-P env: 100% similarity with Chr.14q32.12.