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. 2020 Oct;9(5):1795–1809. doi: 10.21037/tlcr-20-521

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2020 Translational Lung Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Ex vivo expansion of circulating tumour cells. (A) Isolation, expansion and characterization of CTCs from patients with advanced stage NSCLC. Cultured CTCs derived from patient blood in 96 well standard microplate. In well staining of proliferating cells in culture at day 7. Immunofluorescent staining using anti-CK-FITC and anti-CD45-APC. These cells were shown to be CD45 negative. (B) Correlation between baseline CTC count with short term culture success. CTC, circulating tumour cell.