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. 2020 Oct;9(5):1885–1903. doi: 10.21037/tlcr-19-509

Figure 2.

Figure 2

High content screening identifying ACOT11 as an essential gene in promoting lung cancer cell proliferation. (A) Western blotting was performed to determine RNAi efficiency of ACOT11 protein in NCI-H1975 and A549 cell lines 72 h after ACOT11 RNAi lentivirus or control lentivirus was transduced. Using GAPDH as an internal control. ACOT11 was efficiently knocked-down in NCI-1299 and A549 cell lines. (B,C,D,E) High content screening was conducted to measure the effect of ACOT11 knock down on growth of NCI-H1975 (B,C) and A549 (D,E) transduced with GFP-expressing ACOT11 RNAi lentivirus or control lentivirus. Representative images of GFP-expressing cells (successfully transduced cells) was shown in each time point after transduction (B,D), and GFP signaling of transduced cells was normalized to day1 for comparison of growth rate between different groups (C,E). ACOT11 knock down significantly reduced cell growth rate in both NCI-H1975 and A549 lung cancer cell lines. Data are presented as mean ± SD.