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. 2020 Oct;9(5):1885–1903. doi: 10.21037/tlcr-19-509

Figure 3.

Figure 3

ACOT11 knock down inhibit lung cancer cell G1-S transition and induce cell apoptosis in vitro. (A,B,C,D) Colony formation assay was performed to confirm the effect of ACOT11 knock down on cell survival in NCI-H1975 (A,B) and A549 (C,D). Representative images of colony formation for NCI-H1975 (A) and A549 (C) was shown. Colony numbers was calculated in NCI-H1975 (B) and A549 (D) for each group. ACOT11 knock down inhibit colony formation in both NCI-H1975 and A549 lung cancer cell lines. (E,F) Annexin-V-APC labelling and flow cytometry was conducted to measure cell apoptosis in NCI-H1975 (E) after ACOT11 was knocked down. Percentage of cells (F) in apoptosis was quantified. ACOT11 knock down induced cell apoptosis in NCI-H1975. (G,H) PI staining and flow cytometry was performed to evaluate cell cycle in NCI-H1975 (G) after ACOT11 was knocked down. Cell numbers in indicated cell cycle phase was quantified (H). ACOT11 knock down leads to a reduction of cell numbers in S phase and an accumulation of cells in G1 phase in NCI-H1975. Data are presented as mean ± SD. Student’s t-test was conducted to calculate the significance. **P<0.01.