Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct;9(5):1885–1903. doi: 10.21037/tlcr-19-509

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

2020 Translational Lung Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

PMC Copyright notice

Knockdown of ACOT11 significantly inhibit cell migration, invasion and epithelial-mesenchymal transition in vitro. (A,B,C,D) The migration of NCI-H1975 (A,B) and A549 (C,D) was measured by the Transwell migration assay after ACOT11 was knocked down. Representative images of NCI-H1975 (A) and A549 (C) was shown. Cell numbers for NCI-H1975 (B) and A549 (D) were quantified respectively. ACOT11 knocked down inhibit cell migration both in NCI-H1975 and A549. (E,F,G,H) The invasion of NCI-H1975 (E,F) and A549 (G,H) was determined through the Transwell invasion assay after ACOT11 was knocked down. Representative images of NCI-H1975 (E) and A549 (G) was shown. Cell numbers for NCI-H1975 (F) and A549 (H) were quantified respectively. ACOT11 knocked down inhibit cell invasion both in NCI-H1975 and A549. (I) The expression level of EMT marker E-cadherin and vimentin was measured through western blot in NCI-H1975 and A549 cells after ACOT11 was knocked down. ACOT11 knock down increased epithelial cell marker E-cadherin expression and reduced mesenchymal cell marker Vimentin expression. Staining method for (A,C,E,G): 0.5% crystal violet; magnification, ×200. Data are presented as mean ± SD. Student’s t-test was conducted to **P<0.01.