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. 2020 Oct 27;11:604618. doi: 10.3389/fmicb.2020.604618

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Copyright © 2020 Flodman, Corrêa, Dai, Weigele and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Type II restriction of phage T4gt DNA. Phage T4gt gDNA was digested by 10 REases and analyzed by agarose gel electrophoresis (A). 2 log, DNA size marker in 100 bp to 10 kb (NEB). The predicted digestion patterns by NEBcutter are shown in (B). The target recognition sequence and digestion results are summarized in (C). C = complete digestion, P = partial digestion, VP = very partial digestion (only a few weak bands), X = resistant to restriction.