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. 2020 Oct 27;11:604618. doi: 10.3389/fmicb.2020.604618

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Copyright © 2020 Flodman, Corrêa, Dai, Weigele and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Restriction digestion of SP8 gDNA following treatment with 5hmdU DNA kinase. Top panel, restriction of phosphorylated SP8 DNA (SP8-p). Bottom panel, control: restriction of non-p phage SP8 DNA. The phosphorylated DNA appeared largely resistant to restrictions by NcoI (CCATGG), AlwNI (CAGN₃CTG), HpyCH4V (ACGTn), NdeI (CATATG), BbvCI (CCTCAGC), BccI (CTATC), MslI (CAYN₄RTG), NlaIII (CATG), PvuII (CAGCTG), PmlI (CACGTG), NspI (RCATGY), and NsiI (ATGCATn) digestion; and partially resistant to EcoRV (nGATATCn), MlyI (nGAGTCn), MboI (nGATCn), Hpy188I (TCNGA), SapI (nGCTCTTC) digestion. Phosphorylation of 5hmdU had no inhibitory effect on AatII (nGACGTC) and HinfI (nGANTC) digestions. The TG, TC, nG, and Tn dinucleotides in the restriction sites are underlined.