TABLE 1.
Phage | C | 5mCb | 5hmC | 5gmC | 6mAc | 5hmdU |
T4GT7a (Dam+ Dcm+) | >99% | 0.3% | None | None | 0.5% | None |
T4gt | None | None | 83% | 10% α-5gmC 7% β-5gmC | 0.5% | None |
T4 | None | None | None | 60% α-5gmC 40% β-5gmC | 0.4% | None |
Xp12 | None | 100% | None | None | None | None |
SP8 | >99% | None | None | None | None | 100% |
SP8 + 5hmdU DNA kinase | >99% | None | None | None | None | 80% 5hmdU 20% |
phosphorylated-5hmdU | ||||||
Lambda (Dam+ Dcm+) | >99% | 0.4% | None | None | 0.7% | None |
Lambda (Dam– Dcm+) | >99% | 0.4% | None | None | <0.05% | None |
aBacteriophage T4 GT7 [amC87(42–), amE51(56–), NB5060(Δ rllB– denB– ac), unf 39(alc)].
bHost Dcm (CCWGG) partially modified phage T4GT7 and Lambda.
cHost Dam and phage T4 GATC methyltransferase partially modified phage DNA.
Bold values indicate the percentage of base modification.