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. 2020 Oct 27;11:604618. doi: 10.3389/fmicb.2020.604618

TABLE 1.

Percentage of modified cytosine, adenine and 5hmdU bases in phage genomes.

Phage C 5mCb 5hmC 5gmC 6mAc 5hmdU
T4GT7a (Dam+ Dcm+) >99% 0.3% None None 0.5% None
T4gt None None 83% 10% α-5gmC 7% β-5gmC 0.5% None
T4 None None None 60% α-5gmC 40% β-5gmC 0.4% None
Xp12 None 100% None None None None
SP8 >99% None None None None 100%
SP8 + 5hmdU DNA kinase >99% None None None None 80% 5hmdU 20%
phosphorylated-5hmdU
Lambda (Dam+ Dcm+) >99% 0.4% None None 0.7% None
Lambda (Dam Dcm+) >99% 0.4% None None <0.05% None

aBacteriophage T4 GT7 [amC87(42), amE51(56), NB5060(Δ rllB denB ac), unf 39(alc)].

bHost Dcm (CCWGG) partially modified phage T4GT7 and Lambda.

cHost Dam and phage T4 GATC methyltransferase partially modified phage DNA.

Bold values indicate the percentage of base modification.