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. 2020 Oct 27;11:604618. doi: 10.3389/fmicb.2020.604618

TABLE 4.

Digestion of modified phage gDNA with MDREs.

AbaSI (5hmCN20G) (5gmCN20G) FspEI (C5mC) (C5hmC) LpnPI (C5mCDG) (C5hmCDG) D = not C MspJI (5mCNNR) (5hmCNNR) R = A or G McrBC* (R5mC) (R5hmC)
T4gt + ++ ++ ++ + ++ +++
T4 + ++ − − − −
Xp12 ± + + ++ ++ ± ?
T4GT7 − + ± + −

+ + +, positive, complete digestion. + +, positive, nearly complete digestion. +, positive, partial digestion. ±, slight smearing. ± ?, inconclusive (low activity). −, negative, no activity. ∗McrBC requires two sites separated by certain distance (55–3000 bp) and GTP hydrolysis for endonuclease activity.