Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 21;10(10):200227. doi: 10.1098/rsob.200227

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

PMC Copyright notice

Figure 2. — Pluripotent stem cells have elevated expression of CENP-A and CENP-C, and decreased expression of CENP-B. (a) and (b) Cell fractionation experiments to assess total levels of soluble and chromatin bound CENP-A in RPE and hESCs. Immunoblot probed for soluble (sol) and chromatin bound (CB) fractions of CENP-A in RPE and hESCs. Tubulin is used as a marker for the soluble fraction and histone H4K20me2 for the CB fraction (a). Quantification of CENP-A protein levels from six independent experiments (b). (c) Human ESCs, RPE, iPSCs and the fibroblasts they were reprogrammed from, were harvested and processed for SDS-PAGE and immunoblotting. CENP-A, CENP-T, CENP-C and CENP-B levels were assessed with specific antibodies. Tubulin was used as loading control. CENP-A and CENP-E (in figure 4) and CENP-C and CENP-T were detected in the same gel shown using different channels. (d) Quantitation of Western blot bands. The average and standard error of the mean of three replicate experiments are shown. Protein levels were normalized to GAPDH or tubulin.