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. 2020 Nov 3;33(5):108328. doi: 10.1016/j.celrep.2020.108328

Figure 3.

Figure 3

Nr4a1 and Nr4a3 Expression Patterns during T Cell Development in the Thymus

(A) Thymus from Nr4a1-GFP Nr4a3-Tocky mice was analyzed for expression of Nr4a1-GFP and Nr4a3-Timer Blue and Nr4a3-Timer Red expression within live TCRβlo and TCRβhi double positive (DP), CD25− and CD25+ CD4SP, and CD8SP subsets by flow cytometry.

(B–E) Splenic CD4+CD25−, CD4+CD25− T cells (B) and CD8+ T cells (C) were analyzed for expression of Nr4a1-GFP and Nr4a3-Timer Blue and Nr4a3-Timer Red expression by flow cytometry. Summary data of Nr4a1-GFP and Nr4a3-Timer expression in thymic (D) or splenic (E) T cell subsets. Bars represent mean ± SEM; dots represent individual mice; n = 6. Data are pooled from four independent experiments. Statistical analysis by two-way ANOVA with Tukey’s multiple comparisons tests. ***p < 0.001, ****p < 0.0001. See also Figures S3, S4, and S5.