Figure 5.

Peptide Affinity Has Only a Modest Effect on the Threshold for Nr4a3 Activation Compared to Nr4a1

(A) Thymus from OTI Nr4a1-GFP Nr4a3-Tocky mice displaying CD4 versus CD8 (left), CD8 versus TCR-beta in DP cells (middle), or CD8SP (right) populations.

(B) Histogram overlays of Nr4a1-GFP expression (left), Nr4a3-Timer Blue (middle), or Nr4a3-Timer Red (right) in the thymic or splenic populations indicated.

(C) Splenocytes from OTI Nr4a1-GFP Nr4a3-Tocky mice were cultured for 5 h in the presence of a dose range of ova peptide variants (concentrations shown are μM scale). N4 (SIINFEKL), Q4 (SIIQFEKL), or V4 (SIIVFEKL). Summary data of N4 (D), Q4 (E), or V4 (F) responses from (C) for Nr4a1-GFP (green) or Nr4a3-Timer Blue (blue) as raw % (bars represent mean ± SEM) of CD8⁺ T cells (left) or normalized response (right). EC₅₀ values are calculated from curve fitting of normalized data. Data are from two independent experiments.