REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti CD4 AF700 Rat monoclonal RM4-4	BioLegend	Cat# 116022; RRID: AB_2715957
Anti CD4 BUV737 Rat monoclonal GK1.5	BDBiosciences	Cat# 564298; RRID: AB_2738734
Anti TCRVβ 8.1/8.2 PerCP-eFluor710 Rat monoclonal KJ16-133	ThermoFisher	Cat# 46-5813-82; RRID: AB_10549113
Anti TCRVβ 8.1/8.2 BUV395 Rat monoclonal MR5-2	BDBiosciences	Cat# 744335; RRID: AB_2742163
Anti TCRβ AF700 Armenian Hamster monoclonal H57-597	BioLegend	Cat# 109223; RRID: AB_109223
Anti CD69 APC Armenian Hamster monoclonal H1.2F3	BioLegend	Cat# 104513; RRID: AB_492844
Anti CD8 AF700 Rat monoclonal 53-6.7	BioLegend	Cat# 100729; RRID: AB_493702
Anti CD8 BUV395 Rat monoclonal 53-6.7	BDBiosciences	Cat# 563786; RRID: AB_2732919
Anti TCR Vα2 PerCP/Cyanine5.5 Rat monoclonal B20.1	BioLegend	Cat# 127813; RRID: AB_1186118
Anti TCR Vβ5.1/5.2 APC Mouse monoclonal MR9-4	BioLegend	Cat# 139505; RRID: AB_10897800
Anti B220 PerCP-Cy5.5 Rat monoclonal RA3-6B2	BDBiosciences	Cat# 561101; RRID: AB_10565970
Anti CD43 Biotin Rat monoclonal S7	BDBiosciences	Cat# 553269; RRID: AB_2255226
Anti IgM PE-Cy7 Rat monoclonal RMM-1	BioLegend	Cat# 406513; RRID AB_10640069
Anti IgD APC Rat monoclonal 11-26C	Invitrogen	Cat# 17-5993-82; RRID: AB_10598660
Anti CD21 APC Rat monoclonal 7G6	BDBiosciences	Cat# 561770; RRID: AB_10892818
Anti CD23 PE-Cy7 Rat monoclonal B3B4	ThermoFisher	Cat# 25-0232-82; RRID: AB_469604
Anti CD25 PerCP/Cy5.5 Rat monoclonal PC61	BioLegend	Cat# 102029; RRID: AB_893291
Anti CD19 BUV737 Rat monoclonal 1D3	BDBiosciences	Cat# 564296; RRID: AB_2716855
Anti CD3ε Armenian Hamster monoclonal 145-2C11	BioLegend	Cat# 100301; RRID: AB_312666
Chemicals, Peptides, and Recombinant Proteins
Streptavidin BV711	BioLegend	Cat# 405241
MBP Ac1-9[4Y] peptide AcASQYRPSQR	GL Biochem Shanghai	Custom product
N4 OVA 257-264 SIINFEKL peptide	GL Biochem Shanghai	Cat# 181660
Q4 OVA 257-264 SIIQFEKL peptide	GL Biochem Shanghai	Cat# 151560
V4 OVA 257-264 SIIVFEKL peptide	GL Biochem Shanghai	Cat# 151561
Cyclosporin A	Cell Guidance Systems	Cat# SM43; CAS: 59865-13-3
FK506 (Tacrolimus)	Cayman Chemical	Cat# 10007965; CAS: 59865-13-3
PD 0325901	Cayman Chemical	Cat# 13034; CAS: 391210-10-9
DMSO	Sigma Aldrich	Cat# D2650; CAS: 67-68-5
PP2	Sigma Aldrich	Cat# P0042; CAS: 172889-27-9
Critical Commercial Assays
RNeasy mini kit	QIAGEN	Cat# 74104
Invitrogen Superscript IV Reverse Transcriptase	ThermoFisher	Cat# 18090050
Invitrogen Random hexamers	ThermoFisher	Cat# N8080127
Applied Biosystems SYBR green power up master mix	ThermoFisher	Cat# A25752
eFluor-780 fixable viability dye	eBioscience	Cat# 65-0865-14
MoJo Sort nanobeads: CD8 T Cell Isolation Kit	BioLegend	Cat# 480035
MoJo Sort nanobeads: naive CD4 T Cell Isolation Kit	BioLegend	Cat# 480039
Deposited Data
RNA-seq of CA-RIT-NFAT1 CD8 T cells, NFAT1 ChIP-seq of WT and NFAT−/− CD8 T cells	Martinez et al., 2015	GEO: GSE64409
Experimental Models: Organisms/Strains
Mouse: Nr4a3-Tocky founder line 323	Bending et al., 2018b; Obtained from Dr. Masahiro Ono from Imperial College London under MTA	PMID: 29941474
Mouse:Tg4-H2U	Liu et al., 1995; Provided by Prof. David Wraith University of Birmingham	PMID: 7584132
Mouse: IL-10-GFP Tiger	Kamanaka et al., 2006; Provided by Prof. David Wraith University of Birmingham	PMID: 17137799
Mouse: Great-Smart17A	Price et al., 2012; Obtained from Prof. Richard Locksley from University of California, San Francisco under MTA	PMID: 22768117
Mouse: Nr4a1/Nur77-GFP	Moran et al., 2011; Provided by Prof. Graham Anderson University of Birmingham	PMID: 21606508
Mouse: OTI	Charles River Laboratories	Strain Code: 642
Oligonucleotides
Hprt for: AGCCTAAGATGAGCGCAAGT rev: TTACTAGGCAGATGGCCACA	Bending et al., 2018a	PMID: 29991564
Nr4a1 for: TGTGAGGGCTGCAAGGGCTTC rev: AAGCGGCAGAACTGGCAGCGG	Sekiya et al., 2013	PMID: 23334790
Nr4a2 for: CTGTGCGCTGTTTGCGGTGAC rev: CGGCGCTTGTCCACTGGGCAG	Sekiya et al., 2013	PMID: 23334790
Nr4a3 for: AGGGCTTCTTCAAGAGAACGG rev: CCATCCCGACACTGAGACAC	This paper, Designed using NCBI Primer Blast	N/A
Software and Algorithms
GraphPad Prism 7 and 8		https://www.graphpad.com/scientific-software/prism/
FlowJo v10.5.3		https://www.flowjo.com/solutions/flowjo
CyVerse Discovery Environment		https://cyverse.org/discovery-environment
UCSC genome browser		https://genome.ucsc.edu