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. Author manuscript; available in PMC: 2021 Mar 14.
Published in final edited form as: Nat Cell Biol. 2020 Sep 14;22(10):1264–1275. doi: 10.1038/s41556-020-0575-z

Fig. 8: Chemotherapeutic drugs induce pyroptotic cell death via the nPD-L1/GSDMC-mediated non-canonical pathway in breast tumors.

Fig. 8:

(a) Immunoblotting of PD-L1, GSDMC, and cleaved caspase-6 and caspase-8 in MDA-MB-231 cells treated with various chemotherapy drugs as indicated. Blots are representative of three independent experiments. (b and c) Cell death induced by the indicated drugs was determined by LDH release and phase-contrast cell images in MDA-MB-231 cells. Data of LDH-released cell death are shown as mean ± SD of n = 3 biologically independent experiments. Phase-contrast cell images are representative of three independent experiments with similar results. Scale bar, 20 μm. (d and e) Phase-contrast cell images (d) and LDH release (e) in MDA-MB-231 cells treated with the indicated drugs in combination with caspase-6 or caspase-8 inhibition. Data of LDH-released cell death are shown as mean ± SD of n = 3 biologically independent experiments. Phase-contrast cell images are representative of three independent experiments with similar results. Scale bar, 20 μm. P values of all statistical analysis were determined by two-sided Student’s t-test. Statistical source data and unprocessed blots are provided in Source Data Fig. 8.