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. 2020 Oct 15;12(10):6409–6421.

Figure 4.

Figure 4

High glucose induced morphological changes and increased ROS levels in HUVECs. A. HUVECs were incubated with glucose at increasing concentrations (0, 10, 20, and 40 mM) for 24 h. The Cell Counting Kit-8 (CCK-8) assay was performed to detect the cytotoxic effect of glucose. B. HUVECs were incubated with 20 mM glucose for the indicated time. The Cell Counting Kit-8 (CCK-8) assay was performed to detect the cytotoxic effect of glucose. C. The morphology of the cells under light microscopy shows that 40 mM glucose changed the morphology of HUVECs. D, E. The results of the DCFH-DA assay under a fluorescence microscope. Software analysis of the fluorescence density. n≥3 per group, with the experiment repeated three times. Values are the means and standard errors (*P<0.05 and **P<0.01 versus control).