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. 2020 Oct 15;12(10):6220–6234.

Figure 5.

Figure 5

CircRPPH1 functions as a ceRNA by sponging miR-556-5p and indirectly regulating YAP1 expression. A. qRT-PCR and western blot assays were employed to verify the relative expression of YAP1 after circRPPH1 transfection. B. Expression of YAP1 protein was detected by immunofluorescence after circRPPH1 transfection. C. Results of miR-556-5p target analysis using the starBase database. D. Kaplan-Meier overall survival curves according to circRPPH1 expression levels using the starBase database. E and F. qRT-PCR analysis to detect expression of miR-556-5p in breast cancer tissues and cells. G and H. Expression of miR-556-5p and YAP1 was measured after transfection in breast cancer cells. I. RIP assay showing that circRPPH1 was enriched in AGO2-containing miRNAs compared with IgG; miR-556-5p was also detected in the same precipitate. J. Dual-luciferase reporter assay results to test whether circRPPH1 and YAP1 are targets of miR-556-5p.