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. 2020 Oct 15;12(10):6811–6826.

Figure 6.

Figure 6

miRNA inhibitor in SMMC-7721 cells interfered with different exosomes to verify the effect of exosomes of SMMC-7721 cells. A. qPCR confirmation of hsa-miR-24-3p and hsa-miR-21-5p knockdown in different exosomes. ****; P < 0.0001 (vs. exosomes of SMMC-7721 cells); ++++; P < 0.0001 (vs. exosomes of EMT SMMC-7721 cells). B. WB analysis of EMT-related proteins in SMMC-7721 cells with different exosome treatments. C. Migration and invasion analysis of SMMC-7721 cells with different exosome treatments. (miRNA In NC) exo, exosomes of SMMC-7721 cells; EMT (miRNA In NC) exo, exosomes of EMT SMMC-7721 cells; EMT (hsa-miR-24-3p In) exo, exosomes of EMT SMMC-7721 cells with hsa-miR-24-3p knockdown treatment; EMT (hsa-miR-21-5p In) exo, exosomes of EMT SMMC-7721 cells with hsa-miR-21-5p knockdown treatment. All figures are magnified 200 ×.