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. 2020 Oct 15;12(10):6879–6894.

Figure 2.

Figure 2

The mitophagy induced in C2C12 cells by EPS is mediated by AMPK-ULK1. A. Western blotting of AMPK, ULK1 and their phosphorylated forms, with the optical densities of the protein bands being shown to the right (means ± SD (n=6), ##P<0.01 in comparison to the control DMSO group). B. Western blotting of AMPK, ULK1 and their phosphorylated forms and of the indicators of mitochondrial biosynthesis PGC-1α and COX-I in cells exposed to DMSO alone, AICAR alone, or EPS with or without Compound C. C. Western blotting analysis of the indicators of autophagy LC3 and p62. The graph shows means ± SD (n=6). *P<0.05, **P<0.01 in comparison to the control with DMSO group; #P<0.05, ##P<0.01 in comparison to AICAR alone; &P<0.05, &&P<0.01 in comparison to EPS alone; D. Immunofluorescent double-staining shows that COXIV-labeled mitochondria (red) co-localized with LC3 (green) in all of the groups (white arrows). Nuclei are stained blue with DAPI. Scale bar: 25 µm. The quantitative data are means ± SD (n=6). *P<0.05, **P<0.01 in comparison to the control DMSO group; n=6; #P<0.05, ##P<0.01 in comparison to AICAR alone; &P<0.05, &&P<0.01 in comparison to EPS alone.