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. 2020 Oct 15;12(10):6879–6894.

Figure 4.

Figure 4

FUNDC1 is involved in the induction of mitophagy by EPS. A. Western blotting of and the indicators related to autophagy LC3 and p62 in C2C12 cells treated with EPS or FK506. The values presented are means ± SD (n=6). *P<0.05, **P<0.01 in comparison to the control DMSO group; #P<0.05, ##P<0.01 in comparison to treatment with FK506 alone; B. Western blotting of these same proteins in cells treated with EPS with and without FK506. The values presented are means ± SD (n=6). *P<0.05, **P<0.01 in comparison to the control DMSO group; #P<0.05, ##P<0.01 in comparison with EPS alone; C. Immunofluorescent staining of COXIV in mitochondria (red) and co-localized LC3 (green) and FUNDC1 (green or red). Nuclei are stained blue with DAPI. (Scale bar: 25 µm) and quantitative analysis of these co-localizations (means ± SD; n=6). *P<0.05, **P<0.01 in comparison to treatment with FK506 alone; #P<0.05, ##P<0.01 in comparison to treatment with EPS alone.