Skip to main content
. 2020 Oct 15;12(10):6277–6289.

Figure 4.

Figure 4

miR-4644 directly targets and downregulates UBIAD1 expression. (A) RNA pull-down was performed to analyze the interaction between miR-4644 and UBIAD1. **P<0.01 vs. Bio-NC. (B) Sequences of the two putative miR-4644 binding sites in the 3’-UTR of UBIAD1. Luciferase activity in 293T cells co-transfected with wild type (WT) or mutant (Mut1, Mut2, Mut1+Mut2) UBIAD1 3’-UTR luciferase reporter plasmids and miR-4644 mimics or mimic NC. The mRNA (C, E) and protein levels (D, F) of UBIAD1 in T24 and J82 cells following transfection with miR-4644 mimic, miR-4644 inhibitor, and corresponding controls. **P<0.01 vs. mimic NC, ##P<0.01 vs. inhibitor NC. Data are expressed as the mean ± standard deviation (n=3).