KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Myosin heavy chain (chicken) | Developmental Studies Hybridoma Bank | RRID: AB_2147781 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| tbx16 antisense morpholino oligonucleotide 1: AGCCTGCATTATTTAGCCTTCTCTA | Gene tools, LLC | N/A |
| tbx16 antisense morpholino oligonucleotide 2: GATGTCCTCTAAAAGAAAATGTCAG | Gene tools, LLC | N/A |
| Fluorescein Dextran, 10,000 MW, Anionic, Lysine Fixable (Fluoro-Emerald) | Thermo Fisher | D1820 |
| Tetramethylrhodamine Dextran, 10,000 MW, Lysine Fixable (fluoro-Ruby) | Thermo Fisher | D1817 |
| Critical Commercial Assays | ||
| HCR v3.0 | Molecular Instruments | N/A |
| mMessage mMachine SP6 transcription kit | Thermo Fisher | Cat#AM1340 |
| Experimental Models: Organisms/Strains | ||
| Tg(hsp70l:sox2-2A-NLS-KikGR) | Row et al., 2016 | Allele sbu100 |
| Tg(hsp70l:Xla.Tcf.-EGFP) | Martin and Kimelman, 2012 | Allele w74 |
| sox2-2A-sfGFP | Shin et al., 2014 | Allele stl84 |
| Tg(actc1b:gfp) | Higashijima et al., 1997 | Allele zf13 |
| tbx16 | Kimmel et al., 1989 | Allele b104 |
| sox2 | Gou et al., 2018a, 2018b | Allele x50 |
| Tg(neurog1:mKate2-CAAX) | This study | Allele sk100 |
| Oligonucleotides | ||
| tbxta HCR probe pair 1: cctcaacctacctccaacaaGT ATTTCCACCGATTATTATCGGCC | This study | tbxta_B4_odd_1 |
| tbxta HCR probe pair 1: CCCACCGGGCACCCATT CACCGTTCattctcaccatattcgcttc | This study | tbxta_B4_even_1 |
| tbxta HCR probe pair 2: cctcaacctacctccaacaaTTGG CATCGAGGAAAGCTTTGGCAA | This study | tbxta_B4_odd_2 |
| tbxta HCR probe pair 2: GGGACTTCCTTGTGGTCACT TCTCTattctcaccatattcgcttc | This study | tbxta_B4_even_2 |
| tbxta HCR probe pair 3: cctcaacctacctccaacaaCGGA AGAGTTGTCCATGTAGTTATT | This study | tbxta_B4_odd_3 |
| tbxta HCR probe pair 3: ACCAGCTGTC ATGAGACGCAAGACTattctcaccatattcgcttc | This study | tbxta_B4_even_3 |
| tbxta HCR probe pair 4: cctcaacctacct ccaacaaAAGTCCATAACTGCAGCATCAGTCC | This study | tbxta_B4_odd_4 |
| tbxta HCR probe pair 4: TCTAGATTTCCTC CTGAAGCCAAGAattctcaccatattcgcttc | This study | tbxta_B4_even_4 |
| tbxta HCR probe pair 5: cctcaacctacctccaacaaGTT CTACAGAAAGCACATGTAAGAC | This study | tbxta_B4_odd_5 |
| tbxta HCR probe pair 5: CGAAACAGCAAAGTCTGTCT TTCTCattctcaccatattcgcttc | This study | tbxta_B4_even_5 |
| tbx16 HCR probe pair 1: gtccctgcctctatatcttt TGTTAAGTCCAATGCTCTGGTGTTT | This study | tbx16_B3_odd_1 |
| tbx16 HCR probe pair 1: TCTCCATCAGA ACTGGATTAATTCCttccactcaactttaacccg | This study | tbx16_B3_even_1 |
| tbx16 HCR probe pair 2: gtccctgcctctat atctttTTCTGGTACCATGTCCACCAGTAAG | This study | tbx16_B3_odd_2 |
| tbx16 HCR probe pair 2: CTTGTTCCACT TATAT CTCAGACCGttccactcaactttaacccg | This study | tbx16_B3_even_2 |
| tbx16 HCR probe pair 3: gtccctgcctctata tctttCTTTTAGAGTTTGTTCCCTCATCTC | This study | tbx16_B3_odd_3 |
| tbx16 HCR probe pair 3: GGATCAGGCAG ATTTCTGTTTGCCCttccactcaactttaacccg | This study | tbx16_B3_even_3 |
| tbx16 HCR probe pair 4: gtccctgcctctata tctttAAGACTCGGGACTCAAAGCTGGGAC | This study | tbx16_B3_odd_4 |
| tbx16 HCR probe pair 4: TCTGTGTCGGGCACGGTGG CCACGTttccactcaactttaacccg | This study | tbx16_B3_even_4 |
| tbx16 HCR probe pair 5: gtccctgcctctatat ctttCTCACAGTTTGTGTTCACTTTTGGT | This study | tbx16_B3_odd_5 |
| tbx16 HCR probe pair 5: TTCTTGCAAGTGTA GTTAGTGCGTCttccactcaactttaacccg | This study | tbx16_B3_even_5 |
| ttn.1 HCR probe pair 1: cctcaacctacctcca acaaCCTTCCTGACATGTGTCACACGTTC | This study | ttn.1_B4_odd_1 |
| ttn.1 HCR probe pair 1: TCTTCTTCTTTTC CTCCTCACTCTCattctcaccatattcgcttc | This study | ttn.1_B4_even_1 |
| ttn.1 HCR probe pair 2: cctcaacctacctcca acaaAACAGGAATATGCCACTTTGGCTTG | This study | ttn.1_B4_odd_2 |
| ttn.1 HCR probe pair 2: TCTTACAGTCTCG AGGTCATCCTGTattctcaccatattcgcttc | This study | ttn.1_B4_even_2 |
| ttn.1 HCR probe pair 3: cctcaacctacctccaa caaCTTAGGAACAGTTACTGGAGCATAG | This study | ttn.1_B4_odd_3 |
| ttn.1 HCR probe pair 3: TTTTTCTGCCACAGT TGCAGAAGGTattctcaccatattcgcttc | This study | ttn.1_B4_even_3 |
| ttn.1 HCR probe pair 4: cctcaacctacctccaacaaAGTGG CATAATCAGAGGCTTCTCCC | This study | ttn.1_B4_odd_4 |
| ttn.1 HCR probe pair 4: AGAAAATCCACCCCCAGCAA CATCAattctcaccatattcgcttc | This study | ttn.1_B4_even_4 |
| ttn.1 HCR probe pair 5: cctcaacctacctccaa caaTACGGGGAGACTCTGGAGACTTTAC | This study | ttn.1_B4_odd_5 |
| ttn.1 HCR probe pair 5: GAGACCTAATT CCAAATGGTGACTTattctcaccatattcgcttc | This study | ttn.1_B4_even_5 |
| sox2x50 forward genotyping primer: CCAGCAAAGTTACCTCCAACTG | Gou et al., 2018a, 2018b | sox2x50 forward |
| sox2x50 reverse genotyping primer: GCAGGGTGTACTTGTCCTTCTT | Gou et al., 2018a, 2018b | sox2x50 reverse |
| Recombinant DNA | ||
| pCS2+ NLS-kikume | Goto et al., 2017 | N/A |
| pCMV tol2 | Addgene #31823 | |
| Software and Algorithms | ||
| Fiji/ImageJ | Schindelin et al., 2012 | https://fiji.sc |
| Imaris | Bitplane | N/A |
| Metamorph | Molecular Devices | N/A |
| LAS (Leica Application Suite) | Leica Microsystems | N/A |