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. 2020 Nov 9;18:163. doi: 10.1186/s12951-020-00708-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Detection of HUCMSC characteristics. a The representative spindle-like morphology of HUCMSCs in passages 3–5 were observed by microscopy. The multipotency of HUCMSCs for osteogenesis, adipogenesis, and chondrogenesis was determined by alizarin red staining (b), oil-red-o staining (c), and haematoxylin–eosin and alcian blue dual-staining (d), respectively. Scale bar = 500 μm. e Flow cytometric analysis of mesenchymal-related markers: CD29, CD105, CD44, CD45, CD34. ①②③ were cells without antibodies as negative controls, and ④⑤⑥ were flow cytometry results after adding a fluorescent antibody. This experiment was independently repeated three times. f The rates of each marker-positive cells (n = 3)