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. 2020 Nov 10;13:522. doi: 10.1186/s13104-020-05372-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — HPTLC phytochemical profile of Leonotis leonurus var. albiflora Benth. whole flower extract. a L. leonurus var. albiflora Benth. white flower phenotypic appearance (Photo courtesy of Dr B Loedolff) and b its corresponding acetonitrile (75%) extract, HPTLC phytochemical profile. Whole flower metabolite extractions were prepared using various extraction solvents and, the acetonitrile (75%) extracts were selected for downstream analyses. The phytochemical profile was developed fully (mobile phase; chloroform:methanol 9:1, stationary phase; silica_60Å) before reagent visualization (0.1% Fast blue B salt dissolved in 1 mM NaOH) was applied to reveal potential pCB candidates, based solely on an red–orange colour development. Compound x (Rf value: 0.55) represents a colorimetric-specific pCB-like candidate compound which was selected for further LC–MS/MS analyses