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Myograph response to ERα and ERβ ligands on the male and female MCA. Isolated segments of rat middle cerebral artery (MCA), in the myograph. The data are shown as dilation from the myogenic tone. Cumulative concentration-response curves are shown for vehicle (a), 17β-estrogen (b), PPT (c) or DPN (d) added luminally (L) or abluminally (AB). Data points represent means ± SEM. Significance was determined by two-way ANOVA, n = 6. *p < 0.05, **p < 0.005, *** p < 0.001
