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. 2020 Nov 10;11:476. doi: 10.1186/s13287-020-01999-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Histologic analysis of DC capsules before and after marsupialization. a The capsules were detected by hematoxylin-eosin staining (H&E) and Safranin O/Fast Green. Compared with DC capsules before marsupialization (Bm-DCC), DC capsules after marsupialization (Am-DCC) lost the epithelium lining and the fiber became loose. Clumps (which were demonstrated as the bone tissues) were observed in Am-DCC, in which osteocytes (yellow arrows) and osteoblasts (blue arrows) could be seen as well. Boxed areas are shown at higher magnification. Scale bars: 100 μm and 50 μm. b Representative immunofluorescence images of Bm-DCC and Am-DCC. STRO-1-positive cells (yellow arrows) were detected in the fibrous connective tissue of Bm-DCC. STRO-1-positive cells (white triangles) and ALP-positive cells (white arrows) were detected in the fibrous connective tissue of Am-DCC. DAPI (blue) was counterstained to indicate the nucleus. Scale bars: 50 μm