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. 2020 Nov 10;20:1076. doi: 10.1186/s12885-020-07573-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — EP300 KD reduces ABCG2 levels and side population cells. a, Western plot protein quantification of EP300 and ACTB in MDA-MB-231^WT, non-specific scramble shRNA and two stable shRNA EP300 KD clones (n = 1 each). b, EP300 and ABCG2 gene expression in MDA-MB-231 scramble and EP300 KD cells (n = 3 each). c, ABCG2 gene expression after transient siRNA KD of EP300 in three TNBC cell lines (BT20, CAL51) (n = 2 per cell line). d, FACS side population (SP) quantification in the MDA-MB-231^EP300KD (n = 2) and scramble (n = 1)