Table 1.
Primer sequences used for diagnostic testing of Wolbachia
| Test | Gene target | PCR product (bp) | Primer name | Sequence (5ʹ-3ʹ) | References |
|---|---|---|---|---|---|
| Wolbachia presence | 16S rRNA | 438 | W16S-F | CATACCTATTCGAAGGGATAG | [56, 95, 96] |
| W16S-R | TTGCGGGACTTAACCCAACA | ||||
| wsp (General) | 185 | wsp-F | GCATTTGGTTAYAAAATGGACGA | [97] | |
| wsp-R | GGAGTGATAGGCATATCTTCAAT | ||||
| Wolbachia density | RpS3 | 70 | RpS3-F | AGCGTGCCAAGTCGATGAG | [98] |
| RpS3-R | ACGTACTCGTTGCACGGATCTC | ||||
| Supergroup A/B identification | wsp (Supergroup A) | 556 | 136F | TGAAATTTTACCTCTTTT | [65] |
| 691R | AAAAATTAAACGCTACTCCA | ||||
| wsp (Supergroup B) | 442 | 81F | TGGTCCAATAAGTGATGAAGAAAC | ||
| 522R | ACCAGCTTTTGCTTGATA | ||||
| Multilocus sequence typing | gatB | 396 | gatB_F1 | GAKTTAAAYCGYGCAGGBGTT | [57] |
| gatB_R1 | TGGYAAYTCRGGYAAAGATGA | ||||
| coxA | 402 | coxA_F1 | TTGGRGCRATYAACTTTATAG | ||
| coxA_R1 | CTAAAGACTTTKACRCCAGT | ||||
| hcpA | 444 | hcpA_F1 | GAAATARCAGTTGCTGCAAA | ||
| hcpA_R1 | GAAAGTYRAGCAAGYTCTG | ||||
| ftsZ | 435 | ftsZ_F1 | ATYATGGARCATATAAARGATAG | ||
| ftsZ_R1 | TCRAGYAATGGATTRGATAT | ||||
| fbpA | 429 | fbpA_F1 | GCTGCTCCRCTTGGYWTGAT | ||
| fbpA_R1 | CCRCCAGARAAAAYYACTATTC |