Table 1.

Characteristics of the mutational analysis in the CYP21A2 gene

Mutation	Primers	Product of PCR (bp)	Restriction enzyme	Normal	Mutated
Intron 2	P7 P8	378	HhaI	378	24, 354
Pro-30-Leu	P5 P6	249	HhaI	21, 228	249
I172N	P11P2		Taq I	416	394
Deletion 8-bp	P9 P10	89	–	89	81
Gln-318-Stop	P12 P13	136	PstI	25, 111	136