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. 2020 Nov 9;10:19303. doi: 10.1038/s41598-020-75774-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment with PDGF ligands activates both PDGFRs expressed in M28-D5 fibroblasts and 2054E melanoma. Cropped immunoblots show that M28-D5 fibroblasts (a) and 2054E melanoma cells (b) express both PDGFRαα and PDGFRβ. In a time course after treatment with 20 ng/ml PDGF-BB, both receptor isotypes exhibit an increase in high molecular weight signal. Tubulin is used as a loading control. Full-length images of cropped immunoblots are presented in Supplementary Figure S1.