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. 2020 Nov 9;10:19303. doi: 10.1038/s41598-020-75774-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment with PDGF ligands stimulates CDR formation downstream of PDGFR activation. (a) Representative phase contrast images at various time points after PDGF-BB addition. Red arrows in the 12 m images highlight the presence of CDRs. (b,c) Quantification of the frequency of CDRs observed in M28-D5 fibroblasts (b) and 2054E melanoma cells (c) at various times after the addition of PDGF-AA (open circles), PDGF-AB (grey circles) or PDGF-BB (black circles).