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. 2020 Nov 9;10:19303. doi: 10.1038/s41598-020-75774-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR/Cas9 gene editing disrupts PDGFRα expression. (a) Top: Schematic diagram of the mouse Pdgra gene, with exons depicted as boxes and introns as connecting lines. Asterisks indicate the exons targeted for CRISPR/Cas9 cleavage. Bottom: Domain structure of the mouse PDGFRα protein. (b) Cropped immunoblots showing the PDGFR expression profile of both the parental M28-D5 and 2054E cell lines, as well as the Pdgfra^−/− cell lines derived from them. Cells were grown under standard culture conditions. Alpha-tubulin is used as a loading control. Full-length images of the cropped immunoblots are presented in Supplementary Figure S1.