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. 2020 Nov 9;10:19360. doi: 10.1038/s41598-020-76377-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Temporary ligation of the carotid artery provokes consistent sub-occlusive thrombus formation in vivo. Scanning electron microscopy (SEM) images of thrombi (A) 1, (B) 3, (C) 6, (D) 24 and, (E) 72 h after temporary ligation of the carotid artery in control WT mice. (A–E) In all samples sub-occlusive platelet-rich thrombus formation is observed on the injured vessel wall. Damaged endothelium is indicated by a red arrow and undamaged endothelium by a yellow arrow. (F) No platelet adhesion was observed in absence of temporary ligation (3 mm upstream). Representative SEM images of 6 independent ligations are depicted. Scale bar is 80 μm, or 10 μm for zoomed images. (G) Thrombus size was scored by examining SEM images obtained from ligated carotid arteries that were collected at 1, 3, 6, 24 or 72 h after ligation. Scoring scale: 0 = no platelet adhesion, 1 = platelet monolayer, 2 = small thrombi (< 15 layers), and 3 = larger thrombi (> 15 layers).