Table 5.

Sensitivity and specificity of QRDR mutations observed at least in 3 or more isolates and corresponding phenotypic resistance to ciprofloxacin for E. coli isolates. 1. Quinolone resistant-determining regions (QRDR) mutation. Chromosomal mutation gene and location; 2. Percent of isolates presenting the QRDR mutation profile from all isolate with phenotypic resistance to ciprofloxacin. The count of isolates with the QRDR mutation profile is in parenthesis; 3. Number of isolates with the referred QRDR mutation and phenotypic resistance to ciprofloxacin; 4. Number of isolates without the referred QRDR mutation and phenotypic resistance to ciprofloxacin; 5. Number of isolates with the referred QRDR mutation and without phenotypic resistance to ciprofloxacin; 6. Number of isolates without the referred QRDR mutation and without phenotypic resistance to ciprofloxacin; 7. Sensitivity for the referred QRDR mutation to correctly predict phenotypic antimicrobial resistance to ciprofloxacin; 8. Specificity for the absence of the referred QRDR mutation and prediction of lack of phenotypic resistance to ciprofloxacin.

QRDR mutation profile1	% (n) CIP R2	G + : P + 3	G−: P + 4	G + : P−1	G−: P−6	Se7	Sp8
parC_239 gyrA_248 gyrA_259 gyrA_570 parE_1372 gyrA_468 gyrA_273 gyrA_255 gyrA_333 gyrA_300	75.3 (58)	58	20	1	185	74.3	99.5
parC_23 9gyrA_248 gyrA_259 gyrA_468 gyrA_273 gyrA_255 gyrA_333 gyrA_300	18.2 (14)	14	64	1	185	17.9	99.5