Table 1.

Sequence of sgRNA and targeted location on VACV genome.

sgRNA name	Location on VACV genome	sgRNA sequence
E3L_1	48,179–48,152	5′-CUCCGACGAUAUUCCUCCUCGU-3′
E3L_2	48,051–48,078	5′-AAAGACUUAUGAUCCUCUCUCA-3′
E3L_3	47,827–47,854	5′-UAGCUGCAUUAUUUUUAGCAUC-3′
I2L_1	60,828–60,855	5′-AAUACAAAUAUAUCAAUAGUAG-3′
I2L_2	60,881–90,908	5′-AACCAAUACCAACCCCAACAAC-3′
I2L_3	61,026–60,999	5′-AAGUUGUACGCCGCUAUAUUUG-3′
A17L_1	125,654–125,681	5′-GUUUGUUGCAGGUAUACUGUUC-3′
A17L_2	125,761–125,788	5′-UAAGAAAUAAUAUUAAAUAUCU-3′
A17L_3	126,0420–126,069	5′-AUAAUCAUUCAUUCCUCCAUAA-3′
Neg_sgRNA	N/A	5′-AUCUAUUGUUCCGACGUAUUAU-3′