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. 2020 Nov 9;10:19307. doi: 10.1038/s41598-020-76449-9

Table 7.

Q5 SDM primers.

Primer name Melting temperature (°C) Amplification temperature (°C) DNA sequence
N-Terminus Fwd 70 71 5′-AAGCGGAACTACATCCTGGGC-3′
N-Terminus Rev 74 71 5′-GGCCATGGTGGGACCGGT-3′
C-Terminus Fwd 62 58 5′-GGATCCTACCCATACCATG-3′
C-Terminus Rev 57 58 5′-GCCCTTTTTGATGATCTG-3′