Fig. 4. Reduced CCl₄-induced chronic liver injury in AQP3^−/− mice.

a–g CCl₄ (0.5 ml/kg) or vehicle olive oil (OO) was intraperitoneally injected twice a week for 6 weeks. a Hematoxylin and eosin staining of liver from WT and AQP3^−/− mice. Bar, 100 µm. Arrow: infiltrating lymphocytes. b (left) Sirius red staining. Bar, 200 µm. (right) Sirius red-positive staining area (mean ± SE, n = 4 sections from 4 individual mice, **p < 0.01). c–e mRNA expression of indicated genes in liver homogenates by real-time RT-PCR (mean ± SE, n = 4 for olive oil, n = 6 for CCl₄ mice/group, **p < 0.01). Data are expressed as the ratio to 18s RNA. f Ratio of GSH to GSSG in liver homogenate (mean ± SE, n = 4 for olive oil, n = 6 for CCl₄ mice/group *p < 0.05, **p < 0.01). g (left) Phospho-histone H2A.X staining. Bar, 200 µm. (right) Number of phospho-histone H2A.X-positive stained cells (mean ± SE, n = 4 sections from four individual mice, >100 cells from over two different fields from one mouse, **p < 0.01). Statistical analysis for (b)–(g) was performed by two-way ANOVA with Tukey’s multiple comparisons test. Source data, including exact p values, are provided as a Source data file.