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. 2020 Nov 10;15:515. doi: 10.1186/s13018-020-02043-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — HMGA2 is verified to be a target gene of miR-497-5p. a Binding regions between HMGA2 3′UTR and miR-497-5p sequence. b RT-qPCR measurement of HMGA2 mRNA expression in bone tissues of OP patients and controls. c RT-qPCR measurement of HMGA2 mRNA expression during osteogenic differentiation. d RT-qPCR measurement of HMGA2 mRNA expression in MC3T3-E1 cells after transfection. e Western blot measurement of HMGA2 protein expression in MC3T3-E1 cells after transfection. f Luciferase activity of the HMGA2-WT and HMGA2-MT after transfection. g RIP validation of the relationship between miR-497-5p and HMGA2. Data are presented as the mean ± SD of three replicates each. The unpaired t test was used for the comparison between the two groups (b–e); the two-way ANOVA was applied for the comparison among multiple groups (f, g). ^&&p < 0.01 vs. controls; ^%p < 0.05 vs. cells before induction; *p < 0.05 vs. MC3T3-E1 cells transfected with NC mimic; ^{^}p < 0.05 vs. cells treated with Anti-IgG