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. 2020 Nov 10;19:157. doi: 10.1186/s12943-020-01268-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — hsa_circ_0004872 serves as a sponge for miR-224 to inhibit the tumor growth and metastasis of GC in vivo. a The nude mice were subcutaneously injected with BGC-823 cells stably transfected with hsa_circ_0004872 (pLCDH-circ_0004872) (right) or the control BGC-823 cells (pLCDH-circ) (left) in the two flanks of the mice.After 6 days, the subcutaneous tumor size was measured every 3 days and the tumor volume was calculated. b The tumor-bearing mice and the dissected tumors were photographed and shown. A ruler was used to indicate the size of the tumors. c The tumor weight with different cells was shown. d Hematoxylin and eosin (HE) staining was used to detect the tumors. e qRT-PCR analysis of the expression of hsa_circ_0004872 in the tumors. f qRT-PCR analysis of the expression of miR-224 in the tumors. Data were the means ± SD. g IHC analysis of the expression of p21 and Smad4 in the tumors. h Statistical analysis of the percentage of IHC positive exprssion cells in the tumors. i BGC-823 cells stably transfected with hsa_circ_0004872 (pLCDH-circ_0004872) or the control BGC-823 cells (pLCDH-circ) were injected into the tail vein of the nude mice. Six weeks later, the nude mice were dissected. The mice lungs were weighed. j Hematoxylin and eosin (HE) staining of lung tissues was used to detect the metastasis nodules. k The metastatic nodules in the mice lung were counted. All datas were the means ± SD