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. 2020 Nov 9;15:65. doi: 10.1186/s13024-020-00410-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Expression of Dendra2 and TauT4 from a single-copy transgene in C. elegans touch neurons. DIC (a, e) and confocal fluorescent images (b, c, d, f, g, and h) are shown of L4 larval worms expressing single-copy transgenes coding for Dendra2 (b, c, d) or a Dendra2::TauT4 translational fusion (f, g, h). The transgenes are driven by the mec-7 promoter in ALM(L/R) and PLM(L/R) neuron pairs, and also in AVM and PVM neurons, which are not considered further here. Panels c, g and d, h are magnifications of ALM and PLM respectively in the areas encompassed by the white boxes in panels (b and f). Scale bars: 25 μm. ALM is Anterior Lateral Microtubule and PLM is Posterior Lateral Microtubule cells, mechanosensory neurons that mediate behavioral responses to light touch to the body wall within the receptive fields defined by their projections