Table 1.

Quantitative analysis of the viability of the cultured RGC-5 cell line utilizing MTT assay

Group (n = 5 ~ 6)	Control	Pre-OGD vehicle	Pre-OGD Emo 0.25 μM	Pre-OGD Emo 0.5 μM
Cell viability (% of control)	100	38.30 ± 2.51%***	43.81 ± 3.75%	47.52 ± 3.99%†

The value in each group was the ratio of the viability of the cultured RGC-5 cells relative to that of the control group, which was set as 100%. This study include 4 groups including control (normal; RGC-5 cells that were incubated in culture medium containing vehicle), OGD (1 h of pre-OGD administration of vehicle before the OGD condition), Pre-OGD 0.25 μM (1 h pre-OGD administration of 0.25 μM emodin before the OGD condition), and Pre-OGD 0.5 μM (1 h pre-OGD administration of 0.5 μM Emodin). *** denotes an extremely significant difference (P < 0.001) between the normal control and the OGD group. † denotes a significant difference (P = 0.04) between the OGD group and the pre-OGD application of emodin 0.5 μM group. The results are mean ± SE (n = 5 ~ 6). Abbreviations: RGC-5 retinal ganglion cell-5, MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, OGD oxygen glucose deprivation, Emo Emodin