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. 2020 Nov 9;20:511. doi: 10.1186/s12870-020-02710-y

Fig. 4.

Fig. 4

Differential expression of 3 genes in the mapping interval in the parents and NILs. The relative expression levels were calculated by the 2−△△Ct method based on the QU samples, and three replicates were performed for each sample. The housekeeping gene Actin7 was used as the internal control. Values shown are means ± SD (n = 3). Student’s t-test was used to compare QU with ZS11 and BC4F2:3 (AA) with BC4F2:3 (aa). * denotes significance at the probability level of 0.05. ** denotes significance at the probability level of 0.01