Table 2.
Total number of cells recovered and cell type recovery overall is comparable between preservation methods in frozen media (CryoStor® CS10) compared to fresh media (RPMI) in the 18 cell clusters within the 9 cell groupings
| Subject and preservation method | P1 CryoStor |
P1 RPMI |
P2 CryoStor |
P2 RPMI |
P3 CryoStor |
P3 RPMI |
Avg. % cell type per preservation | ||
|---|---|---|---|---|---|---|---|---|---|
| Total number cells recovered | 2576 | 3549 | 1707 | 3846 | 1959 | 1274 | CryoStor | RPMI | |
| Main cell grouping | Clusters | ||||||||
| T/NK cell (%) | 0, 11 | 37 | 19 | 6 | 14 | 15 | 2 | 22 | 14 |
| Epithelial/RBC (%) | 1, 4 | 18 | 5 | 19 | 13 | 25 | 40 | 20 | 14 |
| Macrophages (%) | 2 | 24 | 15 | 0 | 1 | 0 | 0 | 10 | 7 |
| Keratinocytes (%) | 3, 8, 10, 12, 13, 14, 16 | 7 | 48 | 26 | 31 | 22 | 32 | 17 | 38 |
| Epithelial (%) | 5 | 1 | 1 | 24 | 10 | 5 | 6 | 8 | 5 |
| Fibroblast (%) | 6, 17 | 5 | 6 | 5 | 14 | 7 | 0 | 6 | 9 |
| Pericyte (%) | 7, 9 | 6 | 3 | 14 | 13 | 22 | 19 | 13 | 10 |
| Dendritic cells (%) | 15 | 2 | 2 | 4 | 3 | 3 | 0 | 2 | 2 |
| Lymphatic endothelial (%) | 18 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |