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. 2020 Oct 23;20(6):251. doi: 10.3892/etm.2020.9381

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of biochanin A on bone turnover and metabolism. (A) Effects of biochanin A on osteoclast TRAP staining. TRAP⁺ cells are indicated with black arrows. (B) Effects of biochanin A on OCN in the alveolar bone of rats determined by immunohistochemical staining. The black arrows indicate the IHC positive cells. (C) Quantitative analysis of TRAP⁺ cells in five groups. (D) Effects of BA on OCN in serum of rats determined by ELISA. Data are presented as the mean ± SD, n=5 rats per group. ^*P<0.05, ^**P<0.01, and N.S. means no significant difference. TRAP, leukocyte acid phosphatase; BA12.5, 12.5 mg/kg/day biochanin A; BA25, 25 mg/kg/day biochanin A; BA50, 50 mg/kg/day biochanin A.