| Cycling Step | Temperature (°C) | Time | Number of Cycles |
|---|---|---|---|
| Reverse | 50 | 60 min | 1 |
| Transcription | |||
| PCR Enzyme | 95 | 10 min | 1 |
| Activation | |||
| Template | 94 | 30 s | 40 |
| Denaturation | |||
| Annealing/Extension | 55 | 60 s | |
| Droplet Stabilization | 4 | 30 min | 1 |
| Hold (Optional) | 4 | Overnight | 1 |
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