FIG 1.

Comparative analysis of the oncolytic activity of different MRV strains in cancer cell lines. (A) A253, DLD1, and HCT15 cells were infected with T1L, T3D-D, and T3D-L (MOI = 30 PFU/cell). Cells were then collected and stained with propidium iodide (PI) prior to flow cytometry analysis. Cell viability was determined by calculating the ratio of PI-negative cells to total cells. Each value represents an average from triplicate samples. The error bars indicate the standard deviation (SD). Representative results from three independent experiments are shown. Significant differences were determined using two-way ANOVA; ***, P < 0.001. (B and C) Oncolytic activity of T3D-D/T3D-L monoreassortant viruses in cancer cell lines. DLD1 (B) and HCT15 (C) cells were infected with T3D-D/T3D-L monoreassortant viruses at an MOI of 10 PFU/cell. The cells were collected 3 days after infection and viability was measured after PI staining. Each value represents an average from triplicate samples. The error bars indicate the standard deviation. Representative results from two independent experiments are shown. Significance differences were determined by one-way ANOVA; *, P < 0.05; **, P < 0.01; ***, P < 0.001.