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In vitro ExoN activity of MERS-CoV nsp14 mutants. Residues from the DEDDh catalytic motif and ZF1 motif of the nsp14 ExoN domain and the nsp14 N7-MTase domain were mutated as indicated. Assays were performed using a 1:4 ratio between nsp14 and nsp10 and a 90-min incubation at 37°C. For technical details, see the legend to Fig. 7.
