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. 2020 Nov 9;94(23):e01246-20. doi: 10.1128/JVI.01246-20

FIG 3.

FIG 3

A SARS-CoV-2 ExoN knockout mutant is nonviable. (A) Plaque phenotypes of wt SARS-CoV (left) and SARS-CoV-2 (right) and their corresponding ExoN motif I knockout double mutants (DM; D90A/E92A) in Vero E6 cells. Plaque assays were performed using supernatants harvested from transfected cell cultures at 2 dpt for SARS-CoV and 3 dpt for SARS-CoV-2. Samples were diluted 10−6 for SARS-CoV wt, 10−5 for SARS-CoV-2 wt and SARS-CoV DM, and 10−1 for the SARS-CoV-2 DM mutant. (B) Immunolabeling (2 dpt) as described for Fig. 2C but with Vero E6 cells for amplification of SARS-CoV-2 progeny released from BHK-21 cells transfected with wt (top) or DM (bottom) full-length RNA. Bar, 100 μm.