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Impact of ExoN inactivation on intracellular MERS-CoV RNA synthesis. In-gel hybridization analysis of intracellular RNA isolated after 2 or 3 dpt of transfected BHK-21 cells, which were subsequently mixed with HuH7 or Vero cells, as indicated. Purified RNA was separated in an agarose gel and probed with a radiolabeled oligonucleotide probe recognizing the MERS-CoV genome and subgenomic mRNAs.
